Our Platform
Bringing transformative spatial-omics to researchers with microfludics and Next Generation Sequencing
Our Platform DBiT-Seq, can be broken down into three Major Steps
In Situ Chemistry
Spatial Barcoding
Lysis
In situ Chemistry
Biomolecules of interest within the tissue are labeled with a known molecular barcode by adding enzymes, molecular probes, or antibodies through a light permeabilization technique.
Spatial Barcoding
We create a coordinate system within the tissue using two microfluidics chips. Each intersection is a square-shaped element, a pixel, with biomolecules uniquely labeled with Barcode A and Barcode B pairs at a known location
Lysis
The tissue is lysed, and our array of pixels mapping the cellular microenvironment is extracted for library preparation. The library is sequenced and processed using standard single-cell bioinformatics pipelines.
The Platform has enabled higher performance spatial mapping
