Spatial CUT&Tag

Map histone modifications and chromatin associated proteins with spatial CUT&Tag

Spatial CUT&Tag

Scientific diagram of a brain scan with labeled regions and clusters in various colors, titled 'Science' and 'Deng 2022'.

Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level

Yanxiang Deng¹ ², Marek Bartosovic³, Petra Kukanja³, Di Zhang¹, Yang Liu¹ ², Graham Su¹ ², Archibald Enninful¹ ², Zhiliang Bai¹, Gonçalo Castelo-Branco³ ⁴, Rong Fan¹ ² ⁵

Spatial CUT&Tag protocol

Diagram showing a step-by-step process of DNA barcoding with labeled steps 1 through 8, including antibody binding, transposition, barcode ligation, imaging, reverse crosslinking, sequencing, and data analysis.

Target chromatin modifications to map gene regulation

Diagram of an antibody with labeled components including nucleosomes, primary antibody, secondary antibody, histone tail modification, and pA-Tn5.

Anibody validation for sensitivity and specificity

Targets

Validated Targets

  1. Common Post-Translational Modifications (PTMs)

  2. Transcriptional Regulator - CTCF​

  3. Transcription Machinery - RNA Pol II ​

Working With Your Antibody

  • AtlasXomics will work with you to qualify your antibody for spatial CUT&Tag analyses.

  • Note: Antibodies already validated for bulk or single-cell CUT&Tag are generally compatible with our platform.

Validate performance in bulk assay

Graph showing ChIP-seq data with peaks called at Rbfox3 and Cbx8 genes, including signal intensity for IgG negative control, CUT&Tag pseudobulk, and ENCODE ChIP-Seq, with peaks highlighted in blue and red.

Validate specificity in tissue

Microscopic images displaying tissue samples stained with H3K27ac (green) and DAPI (blue). The top row shows a larger tissue sample, and the bottom row shows a different or more magnified section. Labels indicate different staining methods, with the rightmost images combining H3K27ac and DAPI stains.

Map interplay of histone modifications in gene regulation

Diagram of mouse brain tissue showing epigenetic data with charts illustrating chromatin regions and gene markers.