Spatial CUT&Tag

Map histone modifications and chromatin associated proteins with spatial CUT&Tag

Spatial CUT&Tag

Spatial-CUT&Tag: Spatially resolved chromatin modification profiling at the cellular level

Yanxiang Deng¹ ², Marek Bartosovic³, Petra Kukanja³, Di Zhang¹, Yang Liu¹ ², Graham Su¹ ², Archibald Enninful¹ ², Zhiliang Bai¹, Gonçalo Castelo-Branco³ ⁴, Rong Fan¹ ² ⁵

Spatial CUT&Tag protocol

Target chromatin modifications to map gene regulation

Anibody validation for sensitivity and specificity

Targets

Validated Targets

  1. Common Post-Translational Modifications (PTMs)

  2. Transcriptional Regulator - CTCF​

  3. Transcription Machinery - RNA Pol II ​

Working With Your Antibody

  • AtlasXomics will work with you to qualify your antibody for spatial CUT&Tag analyses.

  • Note: Antibodies already validated for bulk or single-cell CUT&Tag are generally compatible with our platform.

Validate performance in bulk assay

Validate specificity in tissue

Map interplay of histone modifications in gene regulation